6Pcs Wide Mouth Mushroom Liquid Culture Lid Grain Spawn Cultivation Mason Jar Lid with 0.2 micron PTFE Syringe Filter & 20mm Self Healing Injection Ports For Mushroom Growing Mycology(Golden)

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Once the grain is fully colonized (meaning the mycelium has completely covered the grain) you can either make more spawn with a grain to grain transfer, or you can add it to a bulk substrate to grow mushrooms right away. This makes it easier when trying to open the lid in a sterile environment without having to pry it off with your fingers.

A few days later I popped them in front of my HEPA and did the usual procedure being more careful since I didn’t have injection ports this time for reason I’ll explain further down the blog. Our UK manufactured, Kilner mason jars are suitable for spawn runs, liquid cultures, BRF cakes or even bottle grows. You’ll see that the top has more perforations/vents which will indicate which way up the filter should be. This will prevent water from the pressure cooker from spilling into your grain and changing the moisture content of the jar.first of all, the grain contains tons of nutrition that is important for fruiting- kind of acts like supplementation. I put them empty, sterilize, than I pour them in a SAB (by the way thanks for the bottle technique out here its awesome !

Please be sure to read your pressure canner’s instructions and familiarize yourself with its safety features BEFORE trying this step.Measure the desired amount of grain, keeping in mind that the rye will expand when moistened and be almost double its original size. I received some of these jars from a friend that knows I like mushroom imagery and am in the middle of rewatching Stranger Things and realized they’re the same jars which makes me love them even more! If you were to use a Syringe filter to inoculate, you would likely filter out much of the mycelium and block up the port but also introduce a good avenue for contamination to ‘wick’ through if that makes sense. Another advantage of this method is that jars can be inoculated with a culture syringe by simply injecting directly through the pillow stuffing.

This is done by performing a grain to grain transfer, were first generation grain spawn is added to additional sterile grain in a ratio between 1:10 and 1:20. Using minimal spore solution and a self healing Injection port ups the chances of succeeding though.I’ve never done this but as long as the fundamental principles of sterile working are followed, I can’t see a reason that it wouldn’t work! As for the agar solution coming through the lid of the bottle- this is likely from “boil over” and it happens when the area around the agar cools down faster than the agar itself, which causes the hot agar solution to boil through the filter.